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GFRA3 Kit ELISA

GFRA3 Reactivité: Humain Colorimetric Sandwich ELISA 16.38-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4883063
  • Antigène Voir toutes GFRA3 Kits ELISA
    GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    16.38-4000 pg/mL
    Seuil minimal de détection
    16.38 pg/mL
    Application
    ELISA
    Fonction
    Human GFR alpha-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human GFR alpha-3.
    Sensibilité
    16 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes GFRA3 Kits ELISA
    GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))
    Autre désignation
    GFR alpha-3 (GFRA3 Produits)
    Synonymes
    GFRA3 Kit ELISA, GFRalpha3 Kit ELISA, Y15110 Kit ELISA, GDNF family receptor alpha 3 Kit ELISA, glial cell line derived neurotrophic factor family receptor alpha 3 Kit ELISA, Gfra3 Kit ELISA, GFRA3 Kit ELISA
    ID gène
    2676
    UniProt
    O60609
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