HMGB3 Kit ELISA

N° du produit ABIN4883200

Détail du produit HMGB3 Kit ELISA

Antigène: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human HMGB3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HMGB3 (High Mobility Group Box 3 (HMGB3))

Autre désignation: HMGB3 (HMGB3 Produits)

Synonymes: HMG-2a Kit ELISA, HMG-4 Kit ELISA, HMG2A Kit ELISA, HMG4 Kit ELISA, Hmg2a Kit ELISA, Hmg4 Kit ELISA, RGD1564407 Kit ELISA, hmgb3 Kit ELISA, MGC54022 Kit ELISA, Xhmgb3 Kit ELISA, MGC88931 Kit ELISA, fa19b06 Kit ELISA, fj43d02 Kit ELISA, wu:fa19b06 Kit ELISA, wu:fj43d02 Kit ELISA, zgc:112073 Kit ELISA, HMGB3 Kit ELISA, NFD03 Kit ELISA, NFD3 Kit ELISA, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR Kit ELISA, high mobility group B3 Kit ELISA, HMG-1 Kit ELISA, HMG2a Kit ELISA, HMGB1 Kit ELISA, high mobility group box 3 Kit ELISA, high mobility group box 3b Kit ELISA, high mobility group box 3 S homeolog Kit ELISA, high mobility group protein Kit ELISA, high mobility group B3 Kit ELISA, High mobility group protein B3 Kit ELISA, HMGB3 Kit ELISA, Hmgb3 Kit ELISA, hmgb3 Kit ELISA, hmgb3b Kit ELISA, hmgb3.S Kit ELISA

ID gène: 3149

UniProt: O15347