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IDO1 Kit ELISA

IDO1 Reactivité: Humain Colorimetric Sandwich ELISA 0.819-200 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4883252
  • Antigène Voir toutes IDO1 Kits ELISA
    IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
    Reactivité
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.819-200 ng/mL
    Seuil minimal de détection
    0.819 ng/mL
    Application
    ELISA
    Fonction
    Human IDO ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human IDO.
    Sensibilité
    0.81 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes IDO1 Kits ELISA
    IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
    Autre désignation
    IDO (IDO1 Produits)
    Synonymes
    INDO Kit ELISA, ido Kit ELISA, indo Kit ELISA, IDO Kit ELISA, cd107b Kit ELISA, IDO1 Kit ELISA, IDO-1 Kit ELISA, Ido Kit ELISA, Indo Kit ELISA, indoleamine 2,3-dioxygenase 1 Kit ELISA, indoleamine 2,3-dioxygenase 1 L homeolog Kit ELISA, indoleamine 2,3-dioxygenase 1-like Kit ELISA, IDO1 Kit ELISA, ido1.L Kit ELISA, ido1 Kit ELISA, Ido1 Kit ELISA, LOC100050681 Kit ELISA
    ID gène
    3620
    UniProt
    P14902
    Pathways
    Activated T Cell Proliferation
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