ING1 Kit ELISA

N° du produit ABIN4883452

Détail du produit ING1 Kit ELISA

Antigène: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human ING1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Autre désignation: ING1 (ING1 Produits)

Synonymes: p24ING1c Kit ELISA, p33 Kit ELISA, p33ING1 Kit ELISA, p33ING1b Kit ELISA, p47 Kit ELISA, p47ING1a Kit ELISA, p33ING1c Kit ELISA, zgc:136918 Kit ELISA, ING1 Kit ELISA, 2610028J21Rik Kit ELISA, AA407184 Kit ELISA, AI875420 Kit ELISA, mING1h Kit ELISA, p33Ing1 Kit ELISA, p37Ing1b Kit ELISA, xing1 Kit ELISA, inhibitor of growth family member 1 Kit ELISA, inhibitor of growth family, member 1 Kit ELISA, inhibitor of growth family member 1 S homeolog Kit ELISA, ING1 Kit ELISA, Ing1 Kit ELISA, ing1 Kit ELISA, ing1.S Kit ELISA

ID gène: 3621

UniProt: Q9UK53

Pathways: Protein targeting to Nucleus, Autophagy