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Integrin beta 5 Kit ELISA
ITGB5
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883484
Détail du produit Integrin beta 5 Kit ELISA
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Antigène
Voir toutes Integrin beta 5 (ITGB5) Kits ELISA
Integrin beta 5 (ITGB5)
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Integrin beta 5 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes .
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Integrin beta 5 (ITGB5) Kits ELISA
Integrin beta 5 (ITGB5)
Autre désignation
Integrin beta 5 (ITGB5 Produits )
Synonymes
ITGB5 Kit ELISA, AA475909 Kit ELISA, AI874634 Kit ELISA, ESTM23 Kit ELISA, [b]-5 Kit ELISA, [b]5 Kit ELISA, [b]5A Kit ELISA, [b]5B Kit ELISA, beta-5 Kit ELISA, beta5 Kit ELISA, RGD1563276 Kit ELISA, integrin, beta 5 Kit ELISA, integrin beta 5 Kit ELISA, integrin subunit beta 5 Kit ELISA, ITGB5 Kit ELISA, Itgb5 Kit ELISA
ID gène
3693
UniProt
P18084
Pathways
Integrin Complex
Vus récemment
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