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PSIP1 Kit ELISA

PSIP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883589
  • Antigène Voir toutes PSIP1 Kits ELISA
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    Reactivité
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human LEDGF ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PSIP1 Kits ELISA
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    Autre désignation
    LEDGF (PSIP1 Produits)
    Synonymes
    PSIP1 Kit ELISA, im:7149861 Kit ELISA, zgc:109898 Kit ELISA, LEDGF Kit ELISA, p75 Kit ELISA, PSIP2 Kit ELISA, DFS70 Kit ELISA, PAIP Kit ELISA, p52 Kit ELISA, AA408851 Kit ELISA, AU015605 Kit ELISA, Dfs70 Kit ELISA, Ledgfa Kit ELISA, Ledgfb Kit ELISA, Psip2 Kit ELISA, Ledgf Kit ELISA, SBP75 Kit ELISA, LEDGF/p75 Kit ELISA, PC4 and SFRS1 interacting protein 1 S homeolog Kit ELISA, PC4 and SFRS1 interacting protein 1 Kit ELISA, PC4 and SFRS1 interacting protein 1a Kit ELISA, LEDGF/p75 transcription factor-like Kit ELISA, psip1.S Kit ELISA, PSIP1 Kit ELISA, psip1a Kit ELISA, psip1 Kit ELISA, Psip1 Kit ELISA
    ID gène
    11168
    UniProt
    O75475
    Pathways
    Chromatin Binding, Ribonucleoprotein Complex Subunit Organization
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