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LGMN Kit ELISA

LGMN Reactivité: Humain Colorimetric Sandwich ELISA 28.7-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4883595
  • Antigène Voir toutes LGMN Kits ELISA
    LGMN (Legumain (LGMN))
    Reactivité
    • 3
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    28.7-7.000 pg/mL
    Seuil minimal de détection
    28.7 pg/mL
    Application
    ELISA
    Fonction
    Human Legumain ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4 (CD244), ADAM-9, Angiopoietin-2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / beta R2, IGF-2, IGF-2 R, IL-1 R6 (IL-1 Rrp2), IL-24, IL-33 (IL-1 F11), Kallikrein 14.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples10 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes LGMN Kits ELISA
    LGMN (Legumain (LGMN))
    Autre désignation
    Legumain (LGMN Produits)
    Synonymes
    AEP Kit ELISA, LGMN1 Kit ELISA, PRSC1 Kit ELISA, AI746452 Kit ELISA, AU022324 Kit ELISA, Prsc1 Kit ELISA, MGC64351 Kit ELISA, cb173 Kit ELISA, cb460 Kit ELISA, sb:cb173 Kit ELISA, zgc:76953 Kit ELISA, wu:fa98g05 Kit ELISA, wu:fj92g08 Kit ELISA, lgmn Kit ELISA, MGC88980 Kit ELISA, DELTA VACUOLAR PROCESSING ENZYME Kit ELISA, DELTAVPE Kit ELISA, delta vacuolar processing enzyme Kit ELISA, legumain Kit ELISA, legumain L homeolog Kit ELISA, delta vacuolar processing enzyme Kit ELISA, Legumain Kit ELISA, LGMN Kit ELISA, Lgmn Kit ELISA, lgmn.L Kit ELISA, lgmn Kit ELISA, DELTA-VPE Kit ELISA, MBOO_RS07785 Kit ELISA, Odosp_3582 Kit ELISA, LOC569153 Kit ELISA
    ID gène
    5641
    UniProt
    Q99538
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
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