LRIG3 Kit ELISA

N° du produit ABIN4883640

Détail du produit LRIG3 Kit ELISA

Antigène: LRIG3 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human LRIG3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human LRIG3

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LRIG3 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))

Autre désignation: LRIG3 (LRIG3 Produits)

Synonymes: LRIG3 Kit ELISA, lrig3 Kit ELISA, GB17149 Kit ELISA, LIG3 Kit ELISA, 9030421L11Rik Kit ELISA, 9130004I02Rik Kit ELISA, 9430095K15Rik Kit ELISA, mKIAA3016 Kit ELISA, RGD1561255 Kit ELISA, leucine rich repeats and immunoglobulin like domains 3 Kit ELISA, leucine-rich repeats and immunoglobulin-like domains 3 Kit ELISA, leucine-rich repeats and immunoglobulin-like domains protein 3 Kit ELISA, leucine-rich repeats and immunoglobulin like domains 3 S homeolog Kit ELISA, leucine-rich repeats and immunoglobulin like domains 3 Kit ELISA, LRIG3 Kit ELISA, lrig3 Kit ELISA, LOC726128 Kit ELISA, lrig3.S Kit ELISA, Lrig3 Kit ELISA

ID gène: 121227

UniProt: Q6UXM1