Lactoferrin Kit ELISA

N° du produit ABIN4883682

Détail du produit Lactoferrin Kit ELISA

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.96-480 ng/mL

Seuil minimal de détection: 1.96 ng/mL

Application: ELISA

Fonction: Human Lactoferrin ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adipsin, Angiogenin, B2M, bIG-H3, CD14, CRP, DPPIV, Endostatin, E-Selectin, FAP, Ferritin, Fetuin A, gp130, HGFR, ICAM-2, IGFBP-2, IGFBP-3, IGFBP-6, Lipocalin-2, L-Selectin, LYVE-1, MCSF R, MMP-9, NCAM-1, Nidogen-1, PAI-1, PF4, RBP4, SAA, TSP-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Autre désignation: Lactoferrin (LTF Produits)

Synonymes: LTF Kit ELISA, GIG12 Kit ELISA, HLF2 Kit ELISA, LF Kit ELISA, Lf Kit ELISA, Csp82 Kit ELISA, MMS10R Kit ELISA, Ms10r Kit ELISA, PLF Kit ELISA, TFEW Kit ELISA, conalbumin Kit ELISA, lactotransferrin Kit ELISA, transferrin Kit ELISA, transferrin (ovotransferrin) Kit ELISA, LTF Kit ELISA, CpipJ_CPIJ000114 Kit ELISA, TF Kit ELISA, Ltf Kit ELISA

ID gène: 4057

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis