nectin-3 Kit ELISA

N° du produit ABIN4883874

Détail du produit nectin-3 Kit ELISA

Antigène: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Nectin-3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Nectin-3. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Autre désignation: Nectin-3 (NECTIN3 Produits)

Synonymes: PVRL3 Kit ELISA, CD113 Kit ELISA, CDW113 Kit ELISA, NECTIN-3 Kit ELISA, PPR3 Kit ELISA, PRR3 Kit ELISA, PVRR3 Kit ELISA, 2610301B19Rik Kit ELISA, 3000002N23Rik Kit ELISA, 4921513D19Rik Kit ELISA, AA407785 Kit ELISA, AU016832 Kit ELISA, AW538082 Kit ELISA, nectin cell adhesion molecule 3 Kit ELISA, poliovirus receptor-related 3 Kit ELISA, Nectin3 Kit ELISA, NECTIN3 Kit ELISA, PVRL3 Kit ELISA, nectin3 Kit ELISA

ID gène: 25945

UniProt: Q9NQS3

Pathways: Cell-Cell Junction Organization