NUCB2 Kit ELISA

N° du produit ABIN4883883

Kit ELISA NUCB2 Humain, Colorimetric test pour la quantification de Humain NUCB2.

Aperçu rapide pour NUCB2 Kit ELISA (ABIN4883883)

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.25 ng/mL - 60 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit NUCB2 Kit ELISA

Seuil minimal de détection: 0.25 ng/mL

Fonction: Human Nesfatin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase- 1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP- 4/RAET1E, UNC5H3, VAMP-1, VAMP-2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Autre désignation: Nesfatin-1

Sujet:

Gene Symbols: NUCB2, NEFA

Protein Name (Synonyms): Nucleobindin-2 (DNA-binding protein NEFA) (Gastric cancer antigen Zg4) (Prepronesfatin) [Cleaved into: Nesfatin-1

ID gène: 4925

UniProt: P80303