MMEL1 Kit ELISA

N° du produit ABIN4883891

Le kit ELISA MMEL1 (ABIN4883891) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain MMEL1.

Aperçu rapide pour MMEL1 Kit ELISA (ABIN4883891)

Antigène: MMEL1 (Membrane Metallo-Endopeptidase-Like 1 (MMEL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.57 ng/mL - 400 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit MMEL1 Kit ELISA

Seuil minimal de détection: 1.57 ng/mL

Fonction: Human Neprilysin-2 (MMEL1) ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human B7-2 (CD86), BAFF R (TNFRSF13C), Calcitonin, Calsyntenin-1, Cathepsin E, cIAP-2 (HIAP-1), Coagulation Factor VII, Complement MASP3, Endocan, EphA2, EphB4, Ephrin-A4, FGF-23, FGF-5, Flt-3 (Flk-2), GLP-1 (7-37 & 7-36- NH2), Glypican 2, GM-CSF R alpha, GP73 (GOLM1), HTRA2 (Omi), IL-20 R alpha, IL-4 R alpha, JAM-C, Luteinizing Hormone (LH), Matrilin-3, Meprin alpha (MEP1A), MSP R (Ron), N-Cadherin, NKp44, Pappalysin-1 (PAPP-A), Parathyroid hormone (PTH), Pepsinogen II (PGC), Peptide YY (PYY), Presenilin 1 (PSEN1), SOX2, TFPI-2, TRACP (ACP5), Trefoil factor 3 (TFF3), Ubiquitin+1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 5 - 50 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: MMEL1 (Membrane Metallo-Endopeptidase-Like 1 (MMEL1))

Autre désignation: Neprilysin-2

Sujet:

Gene Symbols: MELL1, MMEL1, MMEL2, NEP2

Protein Name (Synonyms): Membrane metallo-endopeptidase-like 1 (EC 3.4.24.11) (Membrane metallo-endopeptidase-like 2) (NEP2(m)) (Neprilysin II) (NEPII) (Neprilysin-2) (NEP2) (NL2) [Cleaved into: Membrane metallo-endopeptidase-like 1, soluble form (Neprilysin-2 secreted) (NEP2(s))

ID gène: 79258

UniProt: Q495T6