NCR1 Kit ELISA

N° du produit ABIN4883934

Kit ELISA NCR1 Humain, Colorimetric test pour la quantification de Humain NCR1.

Aperçu rapide pour NCR1 Kit ELISA (ABIN4883934)

Antigène: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.5 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit NCR1 Kit ELISA

Seuil minimal de détection: 4.5 pg/mL

Fonction: Human NKp46/NCR1/CD335 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 5 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Autre désignation: NKp46

Sujet:

Gene Symbols: LY94, NCR1

Protein Name (Synonyms): Natural cytotoxicity triggering receptor 1 (Lymphocyte antigen 94 homolog) (NK cell-activating receptor) (Natural killer cell p46-related protein) (NK-p46) (NKp46) (hNKp46) (CD antigen CD335)

ID gène: 9437

UniProt: O76036

Pathways: Regulation of Leukocyte Mediated Immunity