PCK1 Kit ELISA

N° du produit ABIN4884106

Détail du produit PCK1 Kit ELISA

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human PCK1/PEPCK-C ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1 (PCK1 Produits)

Synonymes: PEPCK-C Kit ELISA, PEPCK1 Kit ELISA, PEPCKC Kit ELISA, PEPCK Kit ELISA, PEPCK-M Kit ELISA, PEPCK2 Kit ELISA, 1810010O14Rik Kit ELISA, 9130022B02Rik Kit ELISA, cb856 Kit ELISA, cb924 Kit ELISA, fj93h11 Kit ELISA, zgc:63869 Kit ELISA, wu:fc51c05 Kit ELISA, wu:fj93h11 Kit ELISA, pepck-c Kit ELISA, pepck1 Kit ELISA, pepckc Kit ELISA, PCK1 Kit ELISA, PPCK1 Kit ELISA, AI265463 Kit ELISA, Pck-1 Kit ELISA, GTP Kit ELISA, PCK Kit ELISA, Pepck Kit ELISA, RATPEPCK Kit ELISA, Ppc1C Kit ELISA, PHOSPHOENOLPYRUVATE CARBOXYKINASE Kit ELISA, T28I19.150 Kit ELISA, T28I19_150 Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, 143299_at Kit ELISA, CG10924 Kit ELISA, CG17725 Kit ELISA, Dmel\\CG17725 Kit ELISA, Dromel_CG17725_FBtr0086701_pepck_mORF Kit ELISA, dPEPCK Kit ELISA, pepck Kit ELISA, PEPC Kit ELISA, PEPCase Kit ELISA, ppc Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, phosphoenolpyruvate carboxykinase 2, mitochondrial Kit ELISA, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Kit ELISA, phosphoenolpyruvate carboxykinase 1 (soluble) Kit ELISA, phosphoenolpyruvate carboxykinase 1 S homeolog Kit ELISA, phosphoenolpyruvate carboxykinase, cytosolic [GTP] Kit ELISA, phosphoenolpyruvate carboxykinase 1, cytosolic Kit ELISA, phosphoenolpyruvate carboxylase 7 Kit ELISA, Phosphoenolpyruvate carboxykinase Kit ELISA, phosphoenolpyruvate carboxylase Kit ELISA, PCK1 Kit ELISA, PCK2 Kit ELISA, Pck2 Kit ELISA, pck1 Kit ELISA, pck1.S Kit ELISA, LOC100634531 Kit ELISA, Pck1 Kit ELISA, pep7 Kit ELISA, Pepck Kit ELISA, LOC107777405 Kit ELISA

ID gène: 5105

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis