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Plexin A4 Kit ELISA

PLXNA4 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884197
  • Antigène Voir toutes Plexin A4 (PLXNA4) Kits ELISA
    Plexin A4 (PLXNA4)
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Plexin A4 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Plexin A4 (PLXNA4) Kits ELISA
    Plexin A4 (PLXNA4)
    Autre désignation
    Plexin A4 (PLXNA4 Produits)
    Synonymes
    D204 Kit ELISA, wu:fd49b01 Kit ELISA, wu:fe15f03 Kit ELISA, PLXNA4A Kit ELISA, FAYV2820 Kit ELISA, PLEXA4 Kit ELISA, PLXNA4B Kit ELISA, PRO34003 Kit ELISA, 9330117B14 Kit ELISA, Plxa4 Kit ELISA, mKIAA1550 Kit ELISA, PLEX2 Kit ELISA, Plxna4 Kit ELISA, PLXNA4 Kit ELISA, plexin A4 Kit ELISA, plexin-A4 Kit ELISA, plxna4 Kit ELISA, PLXNA4 Kit ELISA, LOC100467706 Kit ELISA, Plxna4 Kit ELISA
    ID gène
    91584
    UniProt
    Q9HCM2
    Pathways
    Regulation of Cell Size
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