PRCP Kit ELISA

N° du produit ABIN4884229

Détail du produit PRCP Kit ELISA

Antigène: PRCP (Prolylcarboxypeptidase (PRCP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human PRCP ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PRCP (Prolylcarboxypeptidase (PRCP))

Autre désignation: PRCP (PRCP Produits)

Synonymes: OI13 Kit ELISA, PCOLC Kit ELISA, PCP Kit ELISA, PCP2 Kit ELISA, TLD Kit ELISA, humpcp Kit ELISA, MGC145178 Kit ELISA, 2510048K03Rik Kit ELISA, 2610104A14Rik Kit ELISA, AI451719 Kit ELISA, HUMPCP Kit ELISA, zgc:91816 Kit ELISA, bone morphogenetic protein 1 Kit ELISA, prolylcarboxypeptidase Kit ELISA, lysosomal Pro-X carboxypeptidase Kit ELISA, prolylcarboxypeptidase (angiotensinase C) Kit ELISA, BMP1 Kit ELISA, prcp Kit ELISA, LOC5579822 Kit ELISA, CpipJ_CPIJ006123 Kit ELISA, CpipJ_CPIJ006124 Kit ELISA, Prcp Kit ELISA, PRCP Kit ELISA

ID gène: 5547

UniProt: P42785