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CoREST Kit ELISA

RCOR1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884324
  • Antigène Tous les produits CoREST (RCOR1)
    CoREST (RCOR1) (REST Corepressor 1 (RCOR1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human RCOR1/CoREST ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Tous les produits CoREST (RCOR1)
    CoREST (RCOR1) (REST Corepressor 1 (RCOR1))
    Autre désignation
    RCOR1 (RCOR1 Produits)
    Synonymes
    COREST Kit ELISA, RCOR Kit ELISA, 5730409O11 Kit ELISA, 6720480E22Rik Kit ELISA, AU042633 Kit ELISA, D12Wsu95e Kit ELISA, mKIAA0071 Kit ELISA, rcor1 Kit ELISA, xcoREST Kit ELISA, RCOR1 Kit ELISA, im:6904264 Kit ELISA, si:dkey-46n18.7 Kit ELISA, zgc:158607 Kit ELISA, RGD1305743 Kit ELISA, REST corepressor 1 Kit ELISA, REST corepressor 1 L homeolog Kit ELISA, RCOR1 Kit ELISA, Rcor1 Kit ELISA, rcor1.L Kit ELISA, rcor1 Kit ELISA
    ID gène
    23186
    UniProt
    Q9UKL0
    Pathways
    Regulation of Hormone Metabolic Process, Chromatin Binding
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