TAZ Kit ELISA

N° du produit ABIN4884729

Détail du produit TAZ Kit ELISA

Antigène: TAZ (Tafazzin (TAZ))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human TAZ/WWTR1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TAZ (Tafazzin (TAZ))

Autre désignation: TAZ (TAZ Produits)

Synonymes: wu:fb39f12 Kit ELISA, zgc:91803 Kit ELISA, MGC54019 Kit ELISA, TAZ Kit ELISA, taz Kit ELISA, GB11956 Kit ELISA, Afu2g13960 Kit ELISA, BTHS Kit ELISA, CMD3A Kit ELISA, EFE Kit ELISA, EFE2 Kit ELISA, G4.5 Kit ELISA, LVNCX Kit ELISA, Taz1 Kit ELISA, 5031411C02Rik Kit ELISA, 9130012G04Rik Kit ELISA, AW107266 Kit ELISA, AW552613 Kit ELISA, tafazzin Kit ELISA, tafazzin L homeolog Kit ELISA, tafazzin homolog Kit ELISA, TAZ Kit ELISA, taz Kit ELISA, taz.L Kit ELISA, LOC550948 Kit ELISA, AFUA_2G13960 Kit ELISA, AOR_1_318014 Kit ELISA, Tsp_06712 Kit ELISA, Taz Kit ELISA

ID gène: 25937

UniProt: Q9GZV5