Transferrin Receptor Kit ELISA

N° du produit ABIN4884756

Détail du produit Transferrin Receptor Kit ELISA

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.819-200 ng/mL

Seuil minimal de détection: 0.819 ng/mL

Application: ELISA

Fonction: Human TfR/Transferrin R/CD71 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TfR.

Sensibilité: 0.81 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples200 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: TfR (Transferrin R/CD71) (TFRC Produits)

Synonymes: Trfr Kit ELISA, LOC100219280 Kit ELISA, tfr Kit ELISA, cd71 Kit ELISA, tfr1 Kit ELISA, trfr Kit ELISA, CD71 Kit ELISA, T9 Kit ELISA, TFR Kit ELISA, TFR1 Kit ELISA, TR Kit ELISA, TRFR Kit ELISA, p90 Kit ELISA, 2610028K12Rik Kit ELISA, AI195355 Kit ELISA, AI426448 Kit ELISA, AU015758 Kit ELISA, E430033M20Rik Kit ELISA, Mtvr-1 Kit ELISA, Mtvr1 Kit ELISA, transferrin receptor Kit ELISA, TFRC Kit ELISA, Tfrc Kit ELISA, tfrc Kit ELISA

ID gène: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis