ACP5 Kit ELISA

N° du produit ABIN4884868

Détail du produit ACP5 Kit ELISA

Antigène: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Tartrate Resistant Acid Phosphatase 5 (ACP5 / PAP) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tartrate Resistant Acid Phosphatase 5 (ACP5 / PAP)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Autre désignation: TRACP (ACP5 Produits)

Synonymes: SPENCDI Kit ELISA, TRAP Kit ELISA, acp5 Kit ELISA, sb:cb576 Kit ELISA, zgc:63825 Kit ELISA, wu:fb19f01 Kit ELISA, wu:fb30b03 Kit ELISA, wu:fi14e01 Kit ELISA, wu:fj66f03 Kit ELISA, MGC78938 Kit ELISA, MGC89674 Kit ELISA, TR-AP Kit ELISA, zgc:92339 Kit ELISA, TRACP Kit ELISA, TTRRAP Kit ELISA, Trap Kit ELISA, UF Kit ELISA, acid phosphatase 5, tartrate resistant Kit ELISA, acid phosphatase 5a, tartrate resistant Kit ELISA, acid phosphatase 5, tartrate resistant S homeolog Kit ELISA, tartrate resistant acid phosphatase Kit ELISA, Tartrate-resistant acid phosphatase type 5 Kit ELISA, tartrate-resistant acid phosphatase type 5 Kit ELISA, acid phosphatase 5b, tartrate resistant Kit ELISA, ACP5 Kit ELISA, acp5a Kit ELISA, acp5.S Kit ELISA, acp5 Kit ELISA, NAEGRDRAFT_81291 Kit ELISA, ppa5 Kit ELISA, LOC100282899 Kit ELISA, acp5b Kit ELISA, Acp5 Kit ELISA

ID gène: 54

UniProt: P13686

Pathways: Transition Metal Ion Homeostasis