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UCHL1 Kit ELISA
UCHL1
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.82-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884967
Détail du produit UCHL1 Kit ELISA
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Antigène
Voir toutes UCHL1 Kits ELISA
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Reactivité
Toutes les réactivités sur UCHL1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.82-200 ng/mL
Seuil minimal de détection
0.82 ng/mL
Application
ELISA
Fonction
Human UCH-L1/PGP9.5 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human UCH-L1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes UCHL1 Kits ELISA
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Autre désignation
UCH-L1 (UCHL1 Produits )
Synonymes
PARK5 Kit ELISA, PGP 9.5 Kit ELISA, PGP9.5 Kit ELISA, PGP95 Kit ELISA, Uch-L1 Kit ELISA, UCH-L1 Kit ELISA, UCHL1 Kit ELISA, cb358 Kit ELISA, wu:fc55h08 Kit ELISA, park5 Kit ELISA, pgp9.5 Kit ELISA, uch-l1 Kit ELISA, MGC132191 Kit ELISA, uchl1 Kit ELISA, AW822034 Kit ELISA, C88048 Kit ELISA, R75593 Kit ELISA, UCHL-1 Kit ELISA, gad Kit ELISA, ubiquitin C-terminal hydrolase L1 Kit ELISA, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Kit ELISA, ubiquitin C-terminal hydrolase L1 L homeolog Kit ELISA, ubiquitin carboxyl-terminal esterase L1 Kit ELISA, ubiquitin carboxy-terminal hydrolase L1 Kit ELISA, UCHL1 Kit ELISA, uchl1 Kit ELISA, uchl1.L Kit ELISA, LOC100304750 Kit ELISA, Uchl1 Kit ELISA
ID gène
7345
UniProt
P09936
Pathways
Feeding Behaviour
Vus récemment
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