NAMPT Kit ELISA

N° du produit ABIN4885043

Détail du produit NAMPT Kit ELISA

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Visfatin ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Autre désignation: Visfatin (NAMPT Produits)

Synonymes: 1110035O14Rik Kit ELISA, PBEF Kit ELISA, PBEF1 Kit ELISA, VF Kit ELISA, VISFATIN Kit ELISA, AI314458 Kit ELISA, AI480535 Kit ELISA, NAmPRTase Kit ELISA, Pbef Kit ELISA, Pbef1 Kit ELISA, Visfatin Kit ELISA, visfatin Kit ELISA, pbef Kit ELISA, pbef1 Kit ELISA, nicotinamide phosphoribosyltransferase Kit ELISA, Nicotinamide phosphoribosyltransferase Kit ELISA, nicotinamide phosphoribosyltransferase S homeolog Kit ELISA, NAMPT Kit ELISA, Nampt Kit ELISA, nampt Kit ELISA, Smon_0680 Kit ELISA, Mrub_2845 Kit ELISA, Cseg_3704 Kit ELISA, BC1002_3997 Kit ELISA, Ftrac_2686 Kit ELISA, Varpa_5681 Kit ELISA, Celal_3900 Kit ELISA, Deima_0880 Kit ELISA, BC1001_3724 Kit ELISA, Celly_1696 Kit ELISA, Clole_1542 Kit ELISA, Tsp_05934 Kit ELISA, nampt.S Kit ELISA

ID gène: 10135

UniProt: P43490