YY1 Kit ELISA

N° du produit ABIN4885066

Détail du produit YY1 Kit ELISA

Antigène: YY1 (YY1 Transcription Factor (YY1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human YY1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: YY1 (YY1 Transcription Factor (YY1))

Autre désignation: YY1 (YY1 Produits)

Synonymes: yy1-a Kit ELISA, FIII Kit ELISA, MGC83714 Kit ELISA, yy1l Kit ELISA, zgc:64207 Kit ELISA, YY1 Kit ELISA, LOC494431 Kit ELISA, xyy1 Kit ELISA, delta Kit ELISA, nf-e1 Kit ELISA, ucrbp Kit ELISA, yin-yang-1 Kit ELISA, LOC100304682 Kit ELISA, ino80s Kit ELISA, yy1 Kit ELISA, DELTA Kit ELISA, INO80S Kit ELISA, NF-E1 Kit ELISA, UCRBP Kit ELISA, YIN-YANG-1 Kit ELISA, AW488674 Kit ELISA, fb59g10 Kit ELISA, wu:fa16g07 Kit ELISA, wu:fb59g10 Kit ELISA, YY1 transcription factor L homeolog Kit ELISA, YY1 transcription factor b Kit ELISA, YY1 transcription factor Kit ELISA, transcriptional repressor protein YY1 Kit ELISA, YY1 transcription factor S homeolog Kit ELISA, YY1 transcription factor a Kit ELISA, YY2 transcription factor Kit ELISA, yy1.L Kit ELISA, yy1b Kit ELISA, YY1 Kit ELISA, yy1 Kit ELISA, LOC100304682 Kit ELISA, yy1.S Kit ELISA, Yy1 Kit ELISA, yy1a Kit ELISA, YY2 Kit ELISA

ID gène: 7528

UniProt: P25490