HSP70 Kit ELISA

N° du produit ABIN4885543

Détail du produit HSP70 Kit ELISA

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Mouse Heat Shock Protein 70 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Mouse Heat Shock Protein 70.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Autre désignation: HSP70 (HSP70 Produits)

Synonymes: APG-2 Kit ELISA, HS24/P52 Kit ELISA, HSPH2 Kit ELISA, RY Kit ELISA, hsp70 Kit ELISA, hsp70RY Kit ELISA, CG31354 Kit ELISA, HSP70 Kit ELISA, Hsp70Bb Kit ELISA, hsp70B Kit ELISA, hsp70Bb-prime Kit ELISA, DmelCG5834 Kit ELISA, CG5834 Kit ELISA, HSPA1 Kit ELISA, HSP70B' Kit ELISA, HSPA6 Kit ELISA, ARABIDOPSIS HEAT SHOCK PROTEIN 70 Kit ELISA, ATHSP70 Kit ELISA, heat shock protein 70 Kit ELISA, LOC100305036 Kit ELISA, hsc70 Kit ELISA, Hsp70 Kit ELISA, Hsp70-1 Kit ELISA, Hsp70.1 Kit ELISA, hsp68 Kit ELISA, Hsp110 Kit ELISA, irp94 Kit ELISA, HSP70-2 Kit ELISA, HSPA1B Kit ELISA, HSPA2 Kit ELISA, hsp70-5 Kit ELISA, HSP70-1 Kit ELISA, HSP70.1 Kit ELISA, HSP70.2 Kit ELISA, heat shock protein family A (Hsp70) member 4 Kit ELISA, CG5834 gene product from transcript CG5834-RA Kit ELISA, heat shock protein 70 Kit ELISA, heat shock protein family A (Hsp70) member 6 Kit ELISA, heat shock 70kDa protein 2 Kit ELISA, heat shock 70 kD protein cognate Kit ELISA, Hsp70 family chaperone Kit ELISA, Heat shock protein 70 Kit ELISA, Heat shock protein 70, putative Kit ELISA, heat shock protein 1B Kit ELISA, heat shock protein family A member 4 Kit ELISA, heat shock 70kDa protein 1A Kit ELISA, heat shock protein 1 Kit ELISA, Heat Shock Protein Kit ELISA, heat shock cognate 70-kd protein Kit ELISA, Heat shock 70 kDa protein 1A Kit ELISA, HSPA4 Kit ELISA, Hsp70Bbb Kit ELISA, HSP70 Kit ELISA, HSPA6 Kit ELISA, HSPA2 Kit ELISA, PCC7424_2419 Kit ELISA, Isop_1041 Kit ELISA, CGB_C3390W Kit ELISA, Bacsa_1698 Kit ELISA, dnaK-B Kit ELISA, LOC100305036 Kit ELISA, Hspa1b Kit ELISA, Hspa4 Kit ELISA, HSPA1A Kit ELISA, hsp1 Kit ELISA, hsp-70 Kit ELISA, hsp70 Kit ELISA, LOC108348108 Kit ELISA

ID gène: 15525

UniProt: Q3U2G2