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IL17F Kit ELISA

IL17F Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885567
  • Antigène Voir toutes IL17F Kits ELISA
    IL17F (Interleukin 17F (IL17F))
    Reactivité
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Mouse Interleukin 17F ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Mouse Interleukin 17F.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Ahmad, Attia, Bakheet, Zoheir, Ansari, Korashy, Abdel-Hamied, Ashour, Abd-Allah et al.: "Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-?b, STAT3 and pro-inflammatory mediators and enhancement of I?B? and anti-inflammatory ..." dans: Inflammation, Vol. 38, Issue 2, pp. 846-57, (2015) (PubMed).

    Raeiszadeh Jahromi, Mahesh, Jayaraj, Madhunapantula, Holla, Vishweswaraiah, Ramachandra: "Serum levels of IL-10, IL-17F and IL-33 in patients with asthma: a case-control study." dans: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 51, Issue 10, pp. 1004-13, (2014) (PubMed).

  • Antigène Voir toutes IL17F Kits ELISA
    IL17F (Interleukin 17F (IL17F))
    Autre désignation
    IL-17F (IL17F Produits)
    Synonymes
    CANDF6 Kit ELISA, IL-17F Kit ELISA, ML-1 Kit ELISA, ML1 Kit ELISA, C87042 Kit ELISA, IL17F Kit ELISA, interleukin 17F Kit ELISA, IL17F Kit ELISA, Il17f Kit ELISA
    ID gène
    257630
    UniProt
    Q7TNI7
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
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