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Caveolin-1 Kit ELISA

CAV1 Reactivité: Humain pTyr14 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886011
  • Antigène Voir toutes Caveolin-1 (CAV1) Kits ELISA
    Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))
    Épitope
    pTyr14, total
    Reactivité
    • 8
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-Caveolin-1 (Tyr14) and Total Caveolin-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Caveolin-1 (Tyr14) and Total Caveolin-1 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human Caveolin-1 phosphorylated at site Tyrosine-14 as well as total Caveolin-1.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes Caveolin-1 (CAV1) Kits ELISA
    Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))
    Autre désignation
    Caveolin-1 (CAV1 Produits)
    Synonymes
    fc07c04 Kit ELISA, wu:fc07c04 Kit ELISA, Cav-1 Kit ELISA, bscl3 Kit ELISA, cav1 Kit ELISA, cav1a Kit ELISA, cgl3 Kit ELISA, vip21 Kit ELISA, cav1b Kit ELISA, caveolin-1 Kit ELISA, cav Kit ELISA, mstp085 Kit ELISA, Caveolin-1 Kit ELISA, CAV1 Kit ELISA, cav-1 Kit ELISA, Cav Kit ELISA, caveolin 1 Kit ELISA, BSCL3 Kit ELISA, CGL3 Kit ELISA, MSTP085 Kit ELISA, PPH3 Kit ELISA, VIP21 Kit ELISA, caveolin 1 Kit ELISA, caveolin 1 L homeolog Kit ELISA, caveolin 1 S homeolog Kit ELISA, caveolin 1, caveolae protein Kit ELISA, caveolin 1, caveolae protein, 22kDa Kit ELISA, Caveolin Kit ELISA, cav1 Kit ELISA, cav1.L Kit ELISA, cav1.S Kit ELISA, CAV1 Kit ELISA, Cav1 Kit ELISA, cav-1 Kit ELISA
    ID gène
    857
    UniProt
    Q03135
    Pathways
    Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals
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