ERK1, JNK, P38 Kit ELISA

N° du produit ABIN4886189

Le kit ELISA ERK1, JNK, P38 (ABIN4886189) Humain, Souris et Rat est un kit ELISA Colorimetric conçu pour quantifier Humain, Souris et Rat ERK1, JNK, P38. Ce kit ELISA a été cité dans 2 publications.

Aperçu rapide pour ERK1, JNK, P38 Kit ELISA (ABIN4886189)

Antigène: ERK1, JNK, P38

Épitope: pThr180, pThr183, pThr202, pTyr182, pTyr185, pTyr204

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Lysate

Détail du produit

Fonction: Phospho-ERK/JNK/P38? ELISA Kit. This assay semi-quantitatively measures phophorylated ERK/JNK/P38? in lysate samples.

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes ERK1/2 (T202/Y204), JNK (T183/Y185), P38α (T180/Y182), Total ERK1/2, JNK and P38α in Human, Mouse and Rat Cell Lysates.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Référence

Elamin, Masclee, Troost, Pieters, Keszthelyi, Aleksa, Dekker, Jonkers: "Ethanol impairs intestinal barrier function in humans through mitogen activated protein kinase signaling: a combined in vivo and in vitro approach." dans: PLoS ONE, Vol. 9, Issue 9, pp. e107421, (2015) (PubMed).

Elsherbiny, Abd El Galil, Gabr, Al-Gayyar, Eissa, El-Shishtawy: "Reno-protective effect of NECA in diabetic nephropathy: implication of IL-18 and ICAM-1." dans: European cytokine network, Vol. 23, Issue 3, pp. 78-86, (2012) (PubMed).

Détail du antigène

Antigène: ERK1, JNK, P38

Autre désignation: ERK1, JNK , P38