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SMAD5 Kit ELISA

SMAD5 Reactivité: Humain pSer463 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886297
  • Antigène Voir toutes SMAD5 Kits ELISA
    SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
    Épitope
    pSer463, pSer465
    Reactivité
    • 3
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-SMAD5 (SER463/465) ELISA Kit. This assay semi-quantitatively measures phophorylated SMAD5 (Ser463/465) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human SMAD5 phosphorylated at site Serine-463/465.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes SMAD5 Kits ELISA
    SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
    Autre désignation
    SMAD5 (SMAD5 Produits)
    Synonymes
    DWFC Kit ELISA, JV5-1 Kit ELISA, MADH5 Kit ELISA, Madh5 Kit ELISA, SBN Kit ELISA, fb67b04 Kit ELISA, mad Kit ELISA, madh5 Kit ELISA, sb:cb400 Kit ELISA, smd5 Kit ELISA, wu:fb67b04 Kit ELISA, SMAD5 Kit ELISA, DKFZp468M0619 Kit ELISA, 1110051M15Rik Kit ELISA, AI451355 Kit ELISA, Dwf-C Kit ELISA, MusMLP Kit ELISA, SMAD family member 5 Kit ELISA, SMAD5 Kit ELISA, Smad5 Kit ELISA, smad5 Kit ELISA
    ID gène
    4090
    UniProt
    Q99717
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