PINP Kit ELISA

N° du produit ABIN4947961

Le kit ELISA PINP (ABIN4947961) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PINP. Ce kit ELISA a été cité dans 17 publications.

Aperçu rapide pour PINP Kit ELISA (ABIN4947961)

Antigène: PINP (Procollagen I N-Terminal Propeptide (PINP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 2.47 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit PINP Kit ELISA

Seuil minimal de détection: 2.47 ng/mL

Fonction: ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Sensibilité: < 0.94 ng/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour PINP Kit ELISA (ABIN4947961)

Liu, Li, Liu, Zhu, Xia, Wang, Gu, Wu, Zhang, Liu, Zhou: "Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB signaling pathway." dans: Stem cell research & therapy, Vol. 10, Issue 1, pp. 213, (2020) (PubMed).

Azibani, Pfeffer, Ricke-Hoch, Dowling, Pietzsch, Briton, Baard, Abou Moulig, König, Berliner, Libhaber, Schlothauer, Anthony, Lichtinghagen, Bauersachs, Sliwa, Hilfiker-Kleiner: "Outcome in German and South African peripartum cardiomyopathy cohorts associates with medical therapy and fibrosis markers." dans: ESC heart failure, Vol. 7, Issue 2, pp. 512-522, (2020) (PubMed).

Oezel, Büren, Scholz, Windolf, Windolf: "Effect of antibiotic infused calcium sulfate/hydroxyapatite (CAS/HA) insets on implant-associated osteitis in a femur fracture model in mice." dans: PLoS ONE, Vol. 14, Issue 3, pp. e0213590, (2019) (PubMed).

Malluche, Monier-Faugere, Blomquist, Davenport: "Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors." dans: Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, Vol. 29, Issue 1, pp. 125-134, (2019) (PubMed).

Li, Zhang, Sun, Li, Yu, Fu, Chen: "Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia." dans: Hernia : the journal of hernias and abdominal wall surgery, Vol. 22, Issue 3, pp. 541-547, (2019) (PubMed).

Karakaş, Tulgar Kınık, Özdemir, Muratoğlu Şahin, Tekindal, Haberal: "Congenital Hypothyroidism and Bone Remodeling Cycle." dans: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 2, pp. 106-110, (2018) (PubMed).

Rubiś, Wiśniowska-Smiałek, Wypasek, Rudnicka-Sosin, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Podolec et al.: "12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both ..." dans: Cytokine, Vol. 96, pp. 217-227, (2018) (PubMed).

Liu, Chen, Zhen, Ho, Tsang, Yuen, Lam, Tse, Yiu: "Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus." dans: Biomarkers in medicine, Vol. 11, Issue 7, pp. 569-578, (2018) (PubMed).

Rubiś, Wiśniowska-Śmiałek, Dziewięcka, Rudnicka-Sosin, Kozanecki, Podolec: "Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events." dans: Advances in medical sciences, Vol. 63, Issue 1, pp. 160-166, (2018) (PubMed).

König, Oesser, Scharla, Zdzieblik, Gollhofer: "Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women-A Randomized Controlled Study." dans: Nutrients, Vol. 10, Issue 1, (2018) (PubMed).

Namba, Yamaoka-Tojo, Kakizaki, Nemoto, Fujiyoshi, Hashikata, Kitasato, Hashimoto, Kameda, Meguro, Shimohama, Tojo, Ako: "Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation." dans: Heart and vessels, Vol. 32, Issue 8, pp. 983, (2017) (PubMed).

Anastasilakis, Polyzos, Tsoli, Papatheodorou, Kokkoris, Kaltsas, Terpos, Makras: "Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity." dans: Metabolism: clinical and experimental, Vol. 71, pp. 198-201, (2017) (PubMed).

Rubiś, Wiśniowska-Śmialek, Wypasek, Biernacka-Fijalkowska, Rudnicka-Sosin, Dziewiecka, Faltyn, Khachatryan, Karabinowska, Kozanecki, Tomkiewicz-Pająk, Podolec: "Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy." dans: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 65, Issue 12, pp. 941-949, (2016) (PubMed).

Silk, Greene, Baker, Jander: "The effect of calcium and vitamin D supplementation on bone health of male Jockeys." dans: Journal of science and medicine in sport, Vol. 20, Issue 3, pp. 225-229, (2016) (PubMed).

Sansoni, Vernillo, Perego, Barbuti, Merati, Schena, La Torre, Banfi, Lombardi: "Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners." dans: Endocrine, Vol. 56, Issue 1, pp. 196-204, (2016) (PubMed).

Sokal, Lenarczyk, Kowalski, Mitrega, Pluta, Stabryla-Deska, Streb, Urbanik, Krzeminski, Kalarus: "Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population." dans: Heart rhythm : the official journal of the Heart Rhythm Society, Vol. 13, Issue 5, pp. 1088-95, (2016) (PubMed).

Polyzos, Makras, Anastasilakis, Mintziori, Kita, Papatheodorou, Kokkoris, Terpos: "Periostin and sclerostin levels in juvenile Paget's disease." dans: Clinical cases in mineral and bone metabolism : the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, Vol. 14, Issue 2, pp. 269-271 (PubMed).

Détail du antigène

Antigène: PINP (Procollagen I N-Terminal Propeptide (PINP))

Sujet: P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide

UniProt: P02452