Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Interleukin 17a Kit ELISA

IL17A Reactivité: Souris Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
N° du produit ABIN4986935
  • Antigène Voir toutes Interleukin 17a (IL17A) Kits ELISA
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    Reactivité
    • 18
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.8-500 pg/mL
    Seuil minimal de détection
    7.8 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Natural and recombinant Mouse IL-17A Ligand
    Sensibilité
    4 pg/mL
    Matériel non inclus
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
    Featured
    Discover our best selling IL17A Kit ELISA
    Top Product
    Discover our top product IL17A Kit ELISA
  • Indications d'application
    Detection Wavelength: 450 nm
    Volume d'échantillon
    20 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C
  • Antigène Voir toutes Interleukin 17a (IL17A) Kits ELISA
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    Autre désignation
    IL-17A (IL17A Produits)
    Synonymes
    CTLA8 Kit ELISA, IL-17 Kit ELISA, IL-17A Kit ELISA, IL17 Kit ELISA, Ctla-8 Kit ELISA, Ctla8 Kit ELISA, Il17 Kit ELISA, ChIL-17 Kit ELISA, IL-17F Kit ELISA, IL17A Kit ELISA, CTLA-8 Kit ELISA, interleukin 17A Kit ELISA, IL17A Kit ELISA, Il17a Kit ELISA
    Sujet
    Mouse Interleukin 17 (IL-17, also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold (1-3). Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line (2-5). It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment (5). In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer,and a 45-48 kDa heterodimer with IL-17F (6, 7). Mature mouse IL-17A is 61 % and 89 % aa identical to human and rat IL-17A, respectively (4, 5, 8). While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells (5, 9). Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-γδ T cells, CD1+NK1.1- iNKT cells and CD3-CD4+ LTi-like cells(3, 5, 6, 10-12).A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 13). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30 % aa identity with IL-17RA (14, 15). Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 16).IL-17 is best known for its participation in the recruitment and survival of neutrophils (3, 10, 17,18). Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17(3). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers (17). In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-α, IL-1β and IL-6 production (19).TNF-α and IL-1β then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (20). IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (3, 9). IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (18).
Vous êtes ici:
Support technique