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IL2R Kit ELISA

IL2R Reactivité: Humain Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
N° du produit ABIN4986952
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    IL2R (Interleukin 2 Receptor (IL2R))
    Reactivité
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25-2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Natural and recombinant Human IL-2R Ligand
    Sensibilité
    15 pg/mL
    Matériel non inclus
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
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  • Indications d'application
    Detection Wavelength: 450 nm
    Volume d'échantillon
    20 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C
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    IL2R (Interleukin 2 Receptor (IL2R))
    Autre désignation
    IL-2R (IL2R Produits)
    Sujet
    The biological activities of IL-2 are mediated by its binding to a multi-molecular cellular receptor complex. For several years the receptor was thought to consist of two glycoprotein chains, an alpha chain (IL-2 Rα) and a beta chain (IL-2 Rβ) (1 - 3), which acted together to form a high affinity receptor that transduced the IL-2 signal. IL-2 Rα (also known as Tac antigen and as CD25) is a 55 kDa transmembrane glycoprotein composed of 351 amino acids with only 13 located on the cytoplasmic side of the membrane (4 - 6). The second chain of the complex was cloned in 1989 (7), and is a transmembrane glycoprotein of 575 amino acids (75 kDa), 286 of which are located cytoplasmically and clearly participate in signal transduction (8, 9). Eventually it was discovered that a third chain, IL-2 Rγ, was necessary for high affinity binding, ligand internalization and signalling. Constitutively expressed on many lymphoid cells, it had been overlooked partly because it has no affinity for IL-2 except when IL-2 Rβ is present (7, 10, 11). When cloned, the gene was found to code for a 64 kDa transmembrane protein of 347 amino acids, 84 of which are cytoplasmic (12). Both IL-2 Rβ and IL-2 Rγ are members of the hematopoietin receptor superfamily, whereas IL-2 Rα is related only to the IL-15 R αchain (13 - 15).A model of the IL-2 receptor complex (3, 9, 16 - 21) would describe the high affinity receptor as an αβγ trimer, in which all three chains are in contact with the ligand. Alone, IL-2 Rα binds IL-2 with low affinity, but is unable to transduce a signal. The αβ combination will bind IL-2 with intermediate affinity, but still will not transduce a signal. A βγ complex has intermediate affinity and is capable of signalling if the IL-2 concentration is relatively high. Regardless of many subtleties that determine the affinity of the ligand for the extracellular portions of the receptor components (22 - 24), signalling will ensue if the βand γcytoplasmic domains are brought into close proximity (25 - 27).A soluble form of IL-2 Rα appears in serum, concomitant with its increased expression on cells (18, 28, 29). There are reports of a soluble form of IL-2 Rβ as well (28, 30). The function of the soluble IL-2 Rα is unclear, since it would be expected to be a poor inhibitor of IL-2 because of its low binding affinity. In any case, increased levels of the soluble IL-2 Rα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Results of a number of studies suggest a correlation of levels of IL-2 sRα in serum with the onset of rejection episodes in allograft recipients (18, 31 - 33), with activity of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosis (SLE) (34) and with the course of some leukemias and lymphomas (35 - 40).
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