MMP2 Kit ELISA
Aperçu rapide pour MMP2 Kit ELISA (ABIN5026923)
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Voir toutes MMP2 Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.781 ng/mL
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Fonction
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Sensibilité
- 0.469 ng/mL
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma. Detection Range: 0.781 - 50 ng/mL Sensitivity: < 0.469 ng/mL
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Ingrédients
- plate, standard, diluent, antibodies
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Matériel non inclus
- pipettes, tubes, reader, incubator
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 Reactivité: Humain Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Humain Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Souris Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Cheval Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Porc Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Souris Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Humain Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Porc Colorimetric Sandwich ELISA 47 ng/mL - 3000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Humain Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Plasma, Serum, Tissue Homogenate
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MMP-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MMP-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MMP-2 can be calculated using the standard curve.
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Restrictions
- For Research Use only
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Précaution d'utilisation
- protect your eyes
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Stock
- 4 °C
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Stockage commentaire
- Please refer to the Manual
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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Autre désignation
- MMP-2 (Matrix Metalloproteinase 2)
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Pathways
- Activation of Innate immune Response
Antigène Voir toutes MMP2 Kits ELISA
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