IL-27 Kit ELISA

N° du produit ABIN5026935

Kit ELISA Humain IL-27, test Colorimetric pour la quantification de Humain IL-27.

Aperçu rapide pour IL-27 Kit ELISA (ABIN5026935)

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-27 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human IL-27 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensibilité: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-humanIL-27 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: IL-27 (Interleukin 27)