ALOX15 Kit ELISA

N° du produit ABIN5026944

Kit ELISA Humain ALOX15, test Colorimetric pour la quantification de Humain ALOX15.

Aperçu rapide pour ALOX15 Kit ELISA (ABIN5026944)

Antigène: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit ALOX15 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.

Analytical Method: Quantitative

Sensibilité: < 37.5 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Autre désignation: 15-LO (Arachidonate 15-Lipoxygenase)

Pathways: Regulation of Actin Filament Polymerization