alpha Fetoprotein Kit ELISA

N° du produit ABIN5026955

Le kit ELISA alpha Fetoprotein (ABIN5026955) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain alpha Fetoprotein.

Aperçu rapide pour alpha Fetoprotein Kit ELISA (ABIN5026955)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.313-20 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit alpha Fetoprotein Kit ELISA

Seuil minimal de détection: 0.313 ng/mL

Fonction: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 0.188 ng/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human AFP concentrations in serum, plasma. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate. Standards and samples are pipetted into the wells and AFP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AFP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AFP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: AFP (Alpha Fetoprotein)

Pathways: C21-Steroid Hormone Metabolic Process