IL32 Kit ELISA
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- Antigène Voir toutes IL32 Kits ELISA
- IL32 (Interleukin 32 (IL32))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6-1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-32 concentrations in serum, plasma and other biological fluids.
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensibilité
- < 9.4 pg/mL
- Ingrédients
- plate, standard, diluent, antibodies
- Matériel non inclus
- pipettes, tubes, reader, incubator
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 - 4 h
- Plaque
- Pre-coated
- Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-32 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-32 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-32 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-32 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- protect your eyes
- Stock
- 4 °C
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- Antigène Voir toutes IL32 Kits ELISA
- IL32 (Interleukin 32 (IL32))
- Autre désignation
- IL-32 (Interleukin 32) (IL32 Produits)
- Synonymes
- IL-32alpha Kit ELISA, IL-32beta Kit ELISA, IL-32delta Kit ELISA, IL-32gamma Kit ELISA, NK4 Kit ELISA, TAIF Kit ELISA, TAIFa Kit ELISA, TAIFb Kit ELISA, TAIFc Kit ELISA, TAIFd Kit ELISA, interleukin 32 Kit ELISA, IL32 Kit ELISA
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