Interleukin 35 Kit ELISA

N° du produit ABIN5026966

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Interleukin 35.

Aperçu rapide pour Interleukin 35 Kit ELISA (ABIN5026966)

Antigène: Interleukin 35 (IL35)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Interleukin 35 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensibilité: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-35 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-35 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-35 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-35 can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: Interleukin 35 (IL35)

Autre désignation: IL-35 (Interleukin 35)