PD-L1 Kit ELISA

N° du produit ABIN5026968

Kit ELISA Souris PD-L1, test Colorimetric pour la quantification de Souris PD-L1.

Aperçu rapide pour PD-L1 Kit ELISA (ABIN5026968)

Antigène: PD-L1 (CD274 (PD-L1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit PD-L1 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensibilité: < 0.094 ng/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: PD-L1

Pathways: Cancer Immune Checkpoints