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PD-L1 Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain PD-L1.
N° du produit ABIN5026969

Aperçu rapide pour PD-L1 Kit ELISA (ABIN5026969)

Antigène

Voir toutes PD-L1 Kits ELISA
PD-L1 (CD274 (PD-L1))

Reactivité

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Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

0.156-10 ng/mL

Application

ELISA

Type d'échantillon

Plasma, Serum
  • Seuil minimal de détection

    0.156 ng/mL

    Fonction

    This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.

    Analytical Method

    Quantitative

    Sensibilité

    < 94 pg/mL

    Ingrédients

    plate, standard, diluent, antibodies

    Matériel non inclus

    pipettes, tubes, reader, incubator
  • Volume d'échantillon

    100 μL

    Durée du test

    3 - 4 h

    Plaque

    Pre-coated

    Protocole

    This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Restrictions

    For Research Use only
  • Précaution d'utilisation

    protect your eyes

    Stock

    4 °C
  • Antigène Voir toutes PD-L1 Kits ELISA

    PD-L1 (CD274 (PD-L1))

    Autre désignation

    PD-L1

    Pathways

    Cancer Immune Checkpoints
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