PD-L1 Kit ELISA
Aperçu rapide pour PD-L1 Kit ELISA (ABIN5026969)
Antigène
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.156 ng/mL
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Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
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Analytical Method
- Quantitative
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Sensibilité
- < 94 pg/mL
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Ingrédients
- plate, standard, diluent, antibodies
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Matériel non inclus
- pipettes, tubes, reader, incubator
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Protocole
- This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Précaution d'utilisation
- protect your eyes
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Stock
- 4 °C
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- PD-L1 (CD274 (PD-L1))
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Autre désignation
- PD-L1
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Pathways
- Cancer Immune Checkpoints
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