PD-L1 Kit ELISA
Aperçu rapide pour PD-L1 Kit ELISA (ABIN5026969)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 0.156 ng/mL
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Fonction
- This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate.
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Analytical Method
- Quantitative
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Sensibilité
- 94 pg/mL
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of Human PD-L1 concentrations in serum, plasma. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 94 pg/mL
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Ingrédients
- plate, standard, diluent, antibodies
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Matériel non inclus
- pipettes, tubes, reader, incubator
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Protocole
- This assay employs an antibody specific for Anti-human PD-L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-L1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Précaution d'utilisation
- protect your eyes
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Stock
- 4 °C
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Stockage commentaire
- Please refer to the Manual
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- PD-L1 (CD274 (PD-L1))
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Autre désignation
- PD-L1
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Pathways
- Cancer Immune Checkpoints
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