Melatonin Sulfate Kit ELISA
Aperçu rapide pour Melatonin Sulfate Kit ELISA (ABIN5067888)
Antigène
Tous les produits Melatonin Sulfate (MS)Reactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.
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Analytical Method
- Quantitative
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Sensibilité
- 1 ng/mL
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Attributs du produit
- Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) ELISA Kit
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Ingrédients
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- Microtiter Plate, 1x96 wells
- PAB, 1x200ul
- Melatonin Sulfate (HRP), 40x, 1x200ul
- Standard, 0ng/ml, 1x100ul
- Standard, 1.7ng/ml, 1x100ul
- Standard, 5.2ng/ml, 1x100ul
- Standard, 15.6ng/ml, 1x100ul
- Standard, 46.7ng/ml, 1x100ul
- Standard, 140ng/ml, 1x100ul
- 0: Standard, 420ng/ml, 1x100ul
- 1: Low Control 1, 1x100ul
- 2: High Control 2, 1x100ul
- 3: Assay Buffer, 1x60ml
- 4: Wash Buffer, 20x, 1x50ml
- 5: TMB Substrate, 1x12ml
- 6: Stop Solution, 1M O4, 1x12ml
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Protocole
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Principle:
- Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- 4°C
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- Melatonin Sulfate (MS)
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Autre désignation
- Melatonin Sulfate
Antigène Tous les produits Melatonin Sulfate (MS)
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