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Resistin Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Resistin.
N° du produit ABIN5067898

Aperçu rapide pour Resistin Kit ELISA (ABIN5067898)

Antigène

Voir toutes Resistin (RETN) Kits ELISA
Resistin (RETN)

Reactivité

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Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Application

ELISA

Type d'échantillon

Plasma, Serum, Tissue Culture Medium
  • Fonction

    Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.

    Analytical Method

    Quantitative

    Sensibilité

    0.012 ng/mL

    Attributs du produit

    Resistin, Human, ELISA Kit

    Ingrédients

    • Microtiter Plate, 1x96wells
    • Standard, 1x1vial
    • High Control, 1x1vial
    • Low Control, 1x1vial
    • Pab (Biotin), 1x13ml
    • Streptavidin (HRP), 1x13ml
    • Dilution Buffer, 1x10ml
    • Wash Solution (10X), 1x100ml
    • Substrate Solution (TMB), 1x13ml
    • Stop Solution (O4), 1x13ml
  • Protocole

    Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.

    Restrictions

    For Research Use only
  • Stock

    4 °C

    Stockage commentaire

    4°C
  • Antigène Voir toutes Resistin (RETN) Kits ELISA

    Resistin (RETN)

    Autre désignation

    Resistin

    Pathways

    Feeding Behaviour, Smooth Muscle Cell Migration
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