Resistin Kit ELISA
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- Antigène Voir toutes Resistin (RETN) Kits ELISA
- Resistin (RETN)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
- Type d'échantillon
- Plasma, Serum, Tissue Culture Medium
- Analytical Method
- Quantitative
- Sensibilité
- 0.012 ng/mL
- Attributs du produit
- Resistin, Human, ELISA Kit
- Ingrédients
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- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
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- Protocole
- Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Stockage commentaire
- 4°C
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- Antigène Voir toutes Resistin (RETN) Kits ELISA
- Resistin (RETN)
- Autre désignation
- Resistin (RETN Produits)
- Synonymes
- RETN Kit ELISA, ADSF Kit ELISA, FIZZ3 Kit ELISA, RETN1 Kit ELISA, RSTN Kit ELISA, XCP1 Kit ELISA, Fizz3 Kit ELISA, Rstn Kit ELISA, Xcp4 Kit ELISA, resistin Kit ELISA, RETN Kit ELISA, Retn Kit ELISA
- Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
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