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Resistin Kit ELISA

RETN Reactivité: Humain Colorimetric Sandwich ELISA Plasma, Serum, Tissue Culture Medium
N° du produit ABIN5067898
  • Antigène Voir toutes Resistin (RETN) Kits ELISA
    Resistin (RETN)
    Reactivité
    • 8
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
    Type d'échantillon
    Plasma, Serum, Tissue Culture Medium
    Analytical Method
    Quantitative
    Sensibilité
    0.012 ng/mL
    Attributs du produit
    Resistin, Human, ELISA Kit
    Ingrédients
    • Microtiter Plate, 1x96wells
    • Standard, 1x1vial
    • High Control, 1x1vial
    • Low Control, 1x1vial
    • Pab (Biotin), 1x13ml
    • Streptavidin (HRP), 1x13ml
    • Dilution Buffer, 1x10ml
    • Wash Solution (10X), 1x100ml
    • Substrate Solution (TMB), 1x13ml
    • Stop Solution (O4), 1x13ml
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  • Protocole
    Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    4°C
  • Antigène Voir toutes Resistin (RETN) Kits ELISA
    Resistin (RETN)
    Autre désignation
    Resistin (RETN Produits)
    Synonymes
    RETN Kit ELISA, ADSF Kit ELISA, FIZZ3 Kit ELISA, RETN1 Kit ELISA, RSTN Kit ELISA, XCP1 Kit ELISA, Fizz3 Kit ELISA, Rstn Kit ELISA, Xcp4 Kit ELISA, resistin Kit ELISA, RETN Kit ELISA, Retn Kit ELISA
    Pathways
    Feeding Behaviour, Smooth Muscle Cell Migration
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