Transferrin Receptor Kit ELISA

N° du produit ABIN5067899

Ce kit ELISA Humain Transferrin Receptor est un kit ELISA Colorimetric conçu pour quantifier Humain Transferrin Receptor.

Aperçu rapide pour Transferrin Receptor Kit ELISA (ABIN5067899)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Culture Lysate, Plasma, Serum

Détail du produit Transferrin Receptor Kit ELISA

Fonction: Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.

Analytical Method: Quantitative

Sensibilité: 2 ng/mL

Attributs du produit: Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

Ingrédients:

• Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
• Mab (HRP), 1x13ml
• Standard, 2ug/ml, 1x100ul
• Standard, 1ug/ml, 1x100ul
• Standard, 0.5ug/ml, 1x.100ul
• Standard, 0.2ug/ml, 1x100ul
• Standard, 0.1ug/ml, 1x100ul
• Standard, 0.05ug/ml, 1x100ul
• Quality Control, High, 50X, (5..3ug /ml), 1x50ul
• Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
• Dilution Buffer, 2x13ml
• Wash Solution, 10X, 1x100ml
• Substrate Solution (TMB), 1x13ml
• Stop Solution (0.2M O4), 1x13ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: Transferrin Receptor

Pathways: Transition Metal Ion Homeostasis