Phenylalanine Hydroxylase Kit ELISA

N° du produit ABIN5067907

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Chemical Phenylalanine Hydroxylase.

Aperçu rapide pour Phenylalanine Hydroxylase Kit ELISA (ABIN5067907)

Antigène: Phenylalanine Hydroxylase

Reactivité: Chemical

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit Phenylalanine Hydroxylase Kit ELISA

Fonction: PAH (Phenylalanine Hydroxylase) ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids

Analytical Method: Quantitative

Sensibilité: 46.875 pg/mL

Attributs du produit: PAH (Phenylalanine Hydroxylase) ELISA Kit (Human)

Ingrédients:

• *357883A: Microtiter Strips, 1x96 wells (8x12 wells).
• *357883B: Standard, 2x1 vial
• 357883C: Sample/Standard Dilution Buffer, 1x20ml
• 357883D: Antibody (Biotin) (Concentrated), 1x120ul
• 357883E: Antibody Dilution Buffer, 1x10ml
• 357883F: Streptavidin (HRP) (SABC), 1x120ul
• 357883G: SABC Dilution Buffer, 1x10ml
• 357883H: TMB Substrate, 1x10ml
• 357883J: Stop Solution, 1x10ml
• 357883K: Wash Buffer, 25X, 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: Phenylalanine Hydroxylase

Autre désignation: PAH (Phenylalanine Hydroxylase)

Classe de substances: Chemical