Defensin beta 3 Kit ELISA

N° du produit ABIN5067941

Ce kit ELISA Humain Defensin beta 3 est un kit ELISA Colorimetric conçu pour quantifier Humain Defensin beta 3.

Aperçu rapide pour Defensin beta 3 Kit ELISA (ABIN5067941)

Antigène: Defensin beta 3 (DEFB3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 12.5-400 pg/mL

Application: ELISA

Type d'échantillon: Serum, Cell Culture Samples, Saliva, Tissue Samples

Détail du produit Defensin beta 3 Kit ELISA

Seuil minimal de détection: 12.5 pg/mL

Fonction: The Human Beta Defensin 3 (BD-3) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-3 in cultures of human cells and in appropriately qualified samples from serum, saliva, or other tissue fluids. The kit is suitable for testing a variety of sample types, in accordance with appropriate validation of linearity and recovery.

Analytical Method: Quantitative

Sensibilité: 20 pg/mL

Attributs du produit: Beta Defensin 3, Human, ELISA Kit (BD3)

Ingrédients:

• Microwell Strips, 1x96 wells (12x8-well strips). Coated with purified anti-human BD3 antibodies. Return unused strips to the pouch with desiccant, re-seal and store refrigerated.
• Standard, 400pg/ml, 2x1 vial. Recombinant human BD3. Supplied as a lyophilized powder from buffer, protein stabilizers.
• Ab (Biotin), 100X, 1x150ul. Biotinylated anti-human BD3. Supplied as a liquid in buffer, protein, detergents, 0.05% Bronidox.
• Streptavidin (HRP), 100X, 1x150ul. Supplied as a liquid in buffer with protein, detergents, 0.05% Bronidox.
• Sample Diluent, 10X, 1x10ml.
• Biotin, HRP Diluent, 1x22ml. Supplied as a liquid in buffer, protein, anti-microbials.
• Wash Solution, 100X, 1x10ml.
• TMB Solution, 1x12ml. Chromogenic substrate for HRP containing TMB and peroxide.
• Stop Solution (1% O4), 1x12ml. 1% sulfuric acid. Caution: Corrosive!

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The assay is based on the binding of human Beta Defensin 3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin (HRP). After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-3 in samples is calculated from a standard curve of purified recombinant human BD-3 of designated concentration.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: Defensin beta 3 (DEFB3)

Autre désignation: Beta Defensin 3

Pathways: Production of Molecular Mediator of Immune Response