GFAP Kit ELISA

N° du produit ABIN5067956

Ce kit ELISA Humain GFAP est un kit ELISA Colorimetric conçu pour quantifier Humain GFAP.

Aperçu rapide pour GFAP Kit ELISA (ABIN5067956)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Culture Lysate, Plasma, Serum

Détail du produit GFAP Kit ELISA

Fonction: The Human GFAP ELISA is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of human GFAP in serum, CSF, plasma and tissue culture medium. It is intended for in vitro and research use only.

Analytical Method: Quantitative

Sensibilité: 0.045 ng/mL

Attributs du produit: GFAP, Human, ELISA Kit (Glial Fibrillary Acidic Protein)

Ingrédients:

• Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells
• * Human GFAP Master Calibrator, 1x1 vial
• * Quality Control: High, 6..8ng/ml, 1x1 vial
• * Quality Control: Low, 1..6ng/ml, 1x1 vial
• Pab (Biotin), 1x13ml
• Streptavidin (HRP), 1x13ml
• Standard Diluent, 1x9ml
• Dilution Buffer, 1x13ml
• Wash Solution, 10X, 1x100ml
• TMB Substrate Solution, 1x13ml
• Stop Solution, O4, 1x13ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• In Human GFAP ELISA calibrators or samples are incubated with a rabbit polyclonal anti-human GFAP antibody coated in microtiter wells. After two hours of incubation and a washing, biotin-labeled monoclonal anti-human GFAP antibody is added and incubated with captured GFAP. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution. The absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of GFAP. A standard curve is constructed by plotting absorbance values versus GFAP concentrations of calibrators, and concentrations of unknown samples are determined using this standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP