IgA Kit ELISA

N° du produit ABIN5067962

Ce kit ELISA Humain IgA est un kit ELISA Colorimetric conçu pour quantifier Humain IgA.

Aperçu rapide pour IgA Kit ELISA (ABIN5067962)

Antigène: IgA

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Cell Culture Cells, Serum, Saliva

Détail du produit IgA Kit ELISA

Fonction: The Human IgA ELISA Kit is an in vitro immunoassay for research use in the quantification of human IgA circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of human cells.

Analytical Method: Quantitative

Sensibilité: 20 ng/mL

Attributs du produit: IgA, Human, ELISA Kit

Ingrédients:

• Coated Microwell Plate: 12x8-well strips
• Human IgA Positive Control (ng/ml), 1x 650ul
• Human IgA Standard 50ng/ml, 1x 650ul
• Human IgA Standard 100ng/ml, 1x 650ul
• Human IgA Standard 250ng/ml, 1x 650ul
• Human IgA Standard 500ng/ml, 1x 650ul
• Human IgA Standard 1000ng/ml, 1x 650ul
• IgA (HRP) Conjugate, 100X, 1x 150ul
• Sample Diluent, 20X, 1x10ml
• Wash Solution, 100X, 1x10ml
• TMB Substrate: 1x12ml
• Stop Solution: 1x12ml

Information d'application

Protocole: Principle:

• The Human IgA ELISA kit is based on the binding of human IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: IgA

Classe de substances: Antibody