IgG1 Kit ELISA

N° du produit ABIN5067980

Ce kit ELISA Souris IgG1 est un kit ELISA Colorimetric conçu pour quantifier Souris IgG1.

Aperçu rapide pour IgG1 Kit ELISA (ABIN5067980)

Antigène: IgG1

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Culture Cells, Serum, Saliva

Détail du produit IgG1 Kit ELISA

Fonction: The IgG1 Murine BioAssay ELISA Kit is an in vitro immunoassay for the quantification of IgG1 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.

Analytical Method: Quantitative

Sensibilité: 5 ng/mL

Attributs du produit: IgG1, Mouse, ELISA Kit

Ingrédients:

• Microtiter Plate: 1x96 wells
• Positive Control (ng/ml): 1x650ul
• Standard 5ng/ml, 1x650ul
• Standard 10ng/ml, 1x650ul
• Standard 20ng/ml, 1x650ul
• Standard 40ng/ml, 1x650ul
• Standard 100ng/ml, 1x650ul
• IgG1 (HRP), 100X, 1x150ul
• Sample Diluent, 20X, 1x10ml
• Wash Solution, 100X, 1x10ml
• TMB Substrate, 1x12ml
• Stop Solution, 1x12ml

Information d'application

Protocole: Principle:

• The Mouse IgG1 ELISA kit is based on the binding of mouse IgG1 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG1 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG1 in samples and control is calculated from a curve of standards containing known concentrations of IgG1.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: IgG1

Classe de substances: Antibody