Kynurenine Kit ELISA

N° du produit ABIN5067984

Kit ELISA Kynurenine Chemical, Colorimetric test pour la quantification de Chemical Kynurenine.

Aperçu rapide pour Kynurenine Kit ELISA (ABIN5067984)

Antigène: Kynurenine

Reactivité: Chemical

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit Kynurenine Kit ELISA

Fonction: The Kynurenine (KYN) ELISA Kit is a quantitative competitive assay for the detection of KYN in human serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Sensibilité: > 4.69 pM/mL

Attributs du produit: Kynurenine (KYN) ELISA Kit (Human)

Ingrédients:

• *356970A: Microtiter Strips, 1x96 wells (8x12 wells).
• *356970B: Standard, 2x1 vial
• 356970C: Sample/Standard Dilution Buffer, 1x20ml
• 356970D: Antibody (Biotin) (Concentrated), 1x60ul
• 356970E: Antibody Dilution Buffer, 1x10ml
• 356970F: Streptavidin (HRP) (SABC), 1x120ul
• 356970G: SABC Dilution Buffer, 1x10ml
• 356970H: TMB Substrate, 1x10ml
• 356970J: Stop Solution, 1x10ml
• 356970K: Wash Buffer, 25X, 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with KYN. KYN in the standard or sample competes with a fixed amount of plate-coated KYN for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of KYN in the samples is determined by comparing the absorbance of the samples to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: Kynurenine

Autre désignation: Kynurenine (KYN)

Classe de substances: Chemical